Coding
tphB-6His

Part:BBa_K4378999:Design

Designed by: Leander Maximilian Linke   Group: iGEM22_Uppsala   (2022-09-28)


TphB with His-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 219
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 340


Design Notes

For potential in vitro use of the enzyme an T7-Tag is attached to allow purification through affinity chromatography. Additionally the sequence was codon optimized for E. coli expression


Source

https://www.uniprot.org/uniprotkb/Q3C1E1/entry

References